Journal: Bio-protocol
Article Title: PhosphoLIMBO: An Easy and Efficient Protocol to Separate and Analyze Phospholipids by HPTLC From Plant Material
doi: 10.21769/BioProtoc.5434
Figure Lengend Snippet: Composition of the solvent mix: chloroform, methanol, 2-propanol, KCl 0.25%, methyl acetate, and triethylamine (30:10.2:25:7.8:24:3 v/v/v/v/v/v). Brain PI4,5P2: phosphatidylinositol-4,5-bisphosphate, 18:1. PI3P: phosphatidylinositol-3-phosphate, 18:1. PI4P: phosphatidylinositol-4-phosphate; soybean PS: phosphatidylserine; egg yolk PC: phosphatidylcholine; egg yolk PA: phosphatic acid; egg yolk PE: phosphatidylethanolamine; soybean PI: phosphatidylinositol; egg yolk PG: phosphatidylglycerol. Lipids were stained with primulin 2.5% [PBS 1×, acetone (50:50 v/v)]. N = 7 except for PI3P (n = 4).
Article Snippet: 18:1 phosphatidylinositol-3-phosphate (PI3P) (Avanti polar lipid, catalog number: 850150P) 27.
Techniques: Solvent, Staining