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18:1 pi(3)p  (Avanti Polar)


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    Structured Review

    Avanti Polar 18:1 pi(3)p
    18:1 Pi(3)P, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/18:1 pi(3)p/product/Avanti Polar
    Average 95 stars, based on 85 article reviews
    18:1 pi(3)p - by Bioz Stars, 2026-02
    95/100 stars

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    Composition of the solvent mix: chloroform, methanol, 2-propanol, KCl 0.25%, methyl acetate, and triethylamine (30:10.2:25:7.8:24:3 v/v/v/v/v/v). Brain PI4,5P2: phosphatidylinositol-4,5-bisphosphate, <t>18:1.</t> <t>PI3P:</t> <t>phosphatidylinositol-3-phosphate,</t> 18:1. PI4P: phosphatidylinositol-4-phosphate; soybean PS: phosphatidylserine; egg yolk PC: phosphatidylcholine; egg yolk PA: phosphatic acid; egg yolk PE: phosphatidylethanolamine; soybean PI: phosphatidylinositol; egg yolk PG: phosphatidylglycerol. Lipids were stained with primulin 2.5% [PBS 1×, acetone (50:50 v/v)]. N = 7 except for PI3P (n = 4).
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    Composition of the solvent mix: chloroform, methanol, 2-propanol, KCl 0.25%, methyl acetate, and triethylamine (30:10.2:25:7.8:24:3 v/v/v/v/v/v). Brain PI4,5P2: phosphatidylinositol-4,5-bisphosphate, 18:1. PI3P: phosphatidylinositol-3-phosphate, 18:1. PI4P: phosphatidylinositol-4-phosphate; soybean PS: phosphatidylserine; egg yolk PC: phosphatidylcholine; egg yolk PA: phosphatic acid; egg yolk PE: phosphatidylethanolamine; soybean PI: phosphatidylinositol; egg yolk PG: phosphatidylglycerol. Lipids were stained with primulin 2.5% [PBS 1×, acetone (50:50 v/v)]. N = 7 except for PI3P (n = 4).

    Journal: Bio-protocol

    Article Title: PhosphoLIMBO: An Easy and Efficient Protocol to Separate and Analyze Phospholipids by HPTLC From Plant Material

    doi: 10.21769/BioProtoc.5434

    Figure Lengend Snippet: Composition of the solvent mix: chloroform, methanol, 2-propanol, KCl 0.25%, methyl acetate, and triethylamine (30:10.2:25:7.8:24:3 v/v/v/v/v/v). Brain PI4,5P2: phosphatidylinositol-4,5-bisphosphate, 18:1. PI3P: phosphatidylinositol-3-phosphate, 18:1. PI4P: phosphatidylinositol-4-phosphate; soybean PS: phosphatidylserine; egg yolk PC: phosphatidylcholine; egg yolk PA: phosphatic acid; egg yolk PE: phosphatidylethanolamine; soybean PI: phosphatidylinositol; egg yolk PG: phosphatidylglycerol. Lipids were stained with primulin 2.5% [PBS 1×, acetone (50:50 v/v)]. N = 7 except for PI3P (n = 4).

    Article Snippet: 18:1 phosphatidylinositol-3-phosphate (PI3P) (Avanti polar lipid, catalog number: 850150P) 27.

    Techniques: Solvent, Staining

    The Rf value for each phospholipid was calculated after migration on an HPTLC plate following the PhosphoLIMBO protocol. Rf values for each phospholipid are normalized against the Rf of PC (Rf X /Rf PC ). N = 7 except for PI3P (n = 4). Statistical analysis was conducted by a parametric mixed-effects analysis: P-value = 0.0116 (*) PI4,5P 2 /PI3P; P-value = 0.0212 (*) PI3P/PS; P-value = 0.9992 (ns) PI3P/PI4P; P-value < 0.0001 (****) PI4P/PS; P-value < 0.0001 (****) PS/PC; P-value = 0.0002 (***) PC/PA; P-value = 0.0113 (*) PA/PE; P-value = 0.0015 (**) PE/PI; P-value = 0.0103 (*) PI/PG. The error bars indicate the minimum and maximum values. ns, non-significant.

    Journal: Bio-protocol

    Article Title: PhosphoLIMBO: An Easy and Efficient Protocol to Separate and Analyze Phospholipids by HPTLC From Plant Material

    doi: 10.21769/BioProtoc.5434

    Figure Lengend Snippet: The Rf value for each phospholipid was calculated after migration on an HPTLC plate following the PhosphoLIMBO protocol. Rf values for each phospholipid are normalized against the Rf of PC (Rf X /Rf PC ). N = 7 except for PI3P (n = 4). Statistical analysis was conducted by a parametric mixed-effects analysis: P-value = 0.0116 (*) PI4,5P 2 /PI3P; P-value = 0.0212 (*) PI3P/PS; P-value = 0.9992 (ns) PI3P/PI4P; P-value < 0.0001 (****) PI4P/PS; P-value < 0.0001 (****) PS/PC; P-value = 0.0002 (***) PC/PA; P-value = 0.0113 (*) PA/PE; P-value = 0.0015 (**) PE/PI; P-value = 0.0103 (*) PI/PG. The error bars indicate the minimum and maximum values. ns, non-significant.

    Article Snippet: 18:1 phosphatidylinositol-3-phosphate (PI3P) (Avanti polar lipid, catalog number: 850150P) 27.

    Techniques: Migration, High Performance Thin Layer Chromatography